ISSN 1866-8836
Клеточная терапия и трансплантация

Histological features and blood plasma changes after heterotopic heart transplantation

Nikita V. Grudinin 1, Vadim E. Karev 2, Nikolai S. Bunenkov 1, Vladimir V. Komok 3, Valeria V. Kostevitch 4, Nikolai P. Gorbunov 4, Alexei V. Sokolov 4, Andrey A. Karpov 3, Kirill V. Lepik 3, Alexander N. Shvetsov 3, Youri A. Serov 3, Oleg V. Galibin 3, Alexander S. Nemkov 3, Gennadiy G. Khubulava 3
1 V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow, Russia 2 St. Petersburg Research Institute of Children’s Infections 3 The First Pavlov State Medical University of St. Petersburg 4 St. Petersburg Institute of Experimental Medicine, St. Petersburg, Russia



One of the effective methods for treating severe heart failure is the heart transplantation. On the other hand, the unresolved problem is the rejection of the graft. The study of histological and molecular immune mechanisms of graft rejection is significant.


Objective of the study was to evaluate the histological changes of the graft after heterotopic heart transplantation, as well as changes in the blood plasma of the recipient.

Materials and methods

Two white New Zealand’s rabbits underwent heterotopic heart transplantation, after which a daily measurement of myeloperoxidase in the blood plasma performed. One rabbit received cyclosporine A at a dosage of 10 mg/kg per day and another rabbit was immunosuppressive. The histological examination of transplanted hearts was performed after the rabbits were deceased.


Data on the dynamics of leukocyte myeloperoxidase were obtained after cardiac transplantation and histological changes in the transplanted heart. There are calcification and dystrophy in transplanted heart found in a rabbit without immunosuppression. No signs of graft calcinosis or dystrophy were observed in the cyclosporine-treated animal.


Heterotopic heart transplantation, myeloperoxidase, markers of the graft rejection.

Volume 7, Number 3
09/03/2018 11:39:00 am

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