Experience in high-resolution HLA typing for the bone marrow donor Registry at the 1st State Pavlov Medical University, St. Petersburg
Elena V. Kuzmich, Alexander L. Alyanskiy, Olga Makarenko, Veronika Ermolina, Svetlana Merzlyakova, Svetlana Tyapushkina, Anna Nasredinova, Natalia E. Ivanova, Ludmila S. Zubarovskaya, Boris V. Afanasyev
R. M. Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Chair of Hematology, Transfusiology and Transplantology, Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russian Federation.
Summary
Introduction
The aim of our study was to analyze the frequencies of HLA alleles and five-locus high- resolution HLA haplotypes in the donors examined at the Bone Marrow Donors Registry of the First St. Petersburg State Pavlov Medical University.Materials and methods
The studied cohort included 1,000 donors. High-resolution HLA typing was performed by monoallelic Sanger sequencing using the reagents provided by Protrans (Germany), software was from JSI Medical Systems, Germany (Sequence Pilot version 4.1.2). Data analysis was carried out by Arlequin program version 3.5. The expectation-maximization (EM) algorithm was used for the determination of five-locus haplotype frequencies.Results
Analysis of the high-resolution typing results of 1,000 donors allowed to determine the most frequent HLA alleles and to determine the five-locus high-resolution HLA haplotypes for our Registry, to assess presence of rare HLA alleles, to describe three new HLA alleles. The new HLA alleles were officially named B*44:02:45, DQB1*02:85, DRB1*01:01:30, according to the World Health Organization (WHO) Nomenclature Committee for Factors of the HLA System (November 2016). The rare alleles detected in the studied group were HLA-A*02:460, HLA-A*31:66, HLA-B*08:138. The most frequent HLA alleles in the donors of the registry are presented in Table 1. The most common five-locus high-resolution HLA haplotypes are as follows: A*01:01-B*08:01-C*07:01-DRB1*03:01-DQB1*02:01 (3,4%); A*03:01-B*07:02-C*07:02-DRB1*15:01-DQB1*06:02 (3,1%).
Conclusion
Application of the high resolution typing for definition of HLA phenotypes of the register donors can allow reducing time for the unrelated donor search, and also contributes to detection of the HLA system polymorphisms.Keywords
Bone marrow transplantation, HLA typing, high-resolution, donor registry.