ISSN 1866-8836
Клеточная терапия и трансплантация

DT-09. Prevalence of human herpes viruses 6A and 6B among bone marrow donors

Inara S. Saidullayeva, Mikhail Y. Drokov, Tatyana A. Tupoleva, Dmitriy S. Tikhomirov, Tatiana A. Soldatova, Mikhail A. Demin, Elvira A. Domonova, Maria V. Dovydenko, Ekaterina D. Mikhaltsova, Natalia N. Popova, Zoya V. Konova, Feruza A. Omarova, Elmira I. Kolgaeva, Daria A. Mironova, Luiza A. Karaseva, Olga M. Koroleva, Daria S. Dubnyak, Olga S. Karavaeva, Ulyana V. Maslikova, Vera A. Vasilieva, Larisa A. Kuzmina, Olga S. Pokrovskaya, Elena N. Parovichnikova

National Medical Research Center for Hematology, Moscow, Russia; Central Research Institute of Epidemiology, Moscow, Russia


Contact: Dr. Inara S. Saydullayeva, phone: +7 (495) 614-90-42, e-mail: say-inara@mail.ru

doi 10.18620/ctt-1866-8836-2023-12-3-1-176

Summary

Human herpes viruses 6A and 6B (HHV-6A/B) are widespread in the population and infect representatives of all social groups, starting from early childhood. HHV-6A/B infection is one of the leading causes of complications and mortality in hematologic patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Detection of HHV-6A/B DNA replication in biological fluids of such patients leads to administration of antiviral drugs. There are controversies on such key issues as HHV-6 detection and sufficient viral load of HHV-6A/B DNA required for starting antiviral therapy, as well as criteria of the treatment discontinuation. It is also necessary to consider the toxicity of currently available antiviral drugs. Hence, our aim was to estimate the prevalence of human herpes viruses 6A and 6B among bone marrow donors.

Materials and methods

The study included 51 bone marrow donors (21 men and 30 women) examined from October 2022 to July 2023 at National Medical Research Center for Hematology (Moscow). Biological samples (blood, urine, saliva, oral mucosa scrapings) were collected from all donors before the procedure of PBSC donations. The donors were also interviewed for symptoms of oral mucositis (OMWQ-HN) and cystitis (ACSS). PCR was carried out using methods and reagent kits developed at the Central Research Institute of Epidemiology.

Results and discussion

HHV-6A/B DNA was detected only in saliva (mean concentration of 721 copies) and the probability of detection was 15% (Fig. 1). Based on the interview conducted, no donor showed symptoms of mucositis and cystitis at the time of collection of biological samples.

Conclusion

The obtained data showed that HHV -6A/B DNA in saliva was detected in individuals without clinical manifestations of mucositis, probably because salivary glands are a natural “depot” of the virus. Thus, a probable significance of virus DNA replication in saliva in recipients of allogeneic hematopoietic stem cells is of sufficient relevance.

Keywords

Allogeneic hematopoietic stem cell transplantation, bone marrow donors, human herpesvirus type 6.


Saydullaeva-fig01.jpg

Figure 1. Frequency of detection of HHV-6A/B among the bone marrow donors

Supplement 12-3
09/30/2023

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doi 10.18620/ctt-1866-8836-2023-12-3-1-176

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