IC-04. Influence of HLA donor-recipient compatibility on T-cell immunity restoration in patients after allogeneic stem cell transplantation
Feruza A. Omarova, Natalia N. Popova, Julia O. Davydova, Nikolay M. Kapranov, Mikhail Yu. Drokov, Olga S. Starikova, Ulyana V. Maslikova, Zoya V. Konova, Elmira I. Kolgaeva, Ekaterina D. Mikhaltsova, Mariya V. Dovydenko, Olga M. Koroleva, Anna A. Dmitrova, Darya S. Dubnyak, Mobil I. Akhmedov, Anna S. Garmash, Ksenia A. Nikiforova, Vera A. Vasilyeva, Larisa A. Kuzmina, Irina V. Galtseva, Elena N. Parovichnikova
National Medical Research Center of Hematology, Moscow, Russia
Correspondence:
Dr. Mikhail Yu. Drokov, phone: +7 (495) 614-90-42, e-mail: mdrokov@gmail.com
Summary
Antitumor immunity after allo-HSCT in patients with hematological malignancies is mainly determined by full recovery of T-cell immunity. Influence of various factors on immune system reconstitution in patients after allo-HSCT has been previously studied by many researchers. Our objective is to analyze an influence of HLA donor-recipient compatibility on restoration of T-cell immunity in patients after allo-HSCT.
Patients and methods
The analysis included 34 patients with acute leukemia, who underwent allogeneic transplantation at the National Research Center for Hematology in 2019-2021. Reduced-intensity conditioning regimen was used in 33 patients, myeloablative – in 1. Post-transplant Cyclophosphamide was used for GVHD prophylaxis, and peripheral blood stem cells were used as a graft source in all patients. Three patients underwent allo-HSCT from unrelated HLA-mismatched donors, 9 – from full matched unrelated, and 22 – from haploidentical donors. The peripheral blood samples were used on day +30, +60, +90, +180 to analyze reconstitution of T memory cells (CD8 + / CD4 +: Tnv + Tscm, Tcm, Ttm, Tem, Tte). The Kruskal-Wallis test was used to determine an impact of a donor type on T cell reconstitution.
Results
There were no statistical differences in reconstitution of memory T-cell subpopulations between the groups (p >0.05).
Conclusion
Thus, it was proved that HLA donor-recipient compatibility does not affect T cell immune reconstitution during the first 6 months after allo-HSCT in patients with acute leukemia.
Keywords
Allogeneic hematopoietic stem cell transplantation, T-cell immunity.
Figure 1. Reconstitution of CD8+ memory cells subpopulations
Figure 2. Reconstitution of CD4+ memory cells subpopulations
